OTO method for preservation of actin filaments in electron microscopy.

Actin - like filaments and membrane rearrangement in oxyntic cells ( heavy meromyosin / membrane translocation / membrane - associated filaments / HCl secretion / electron microscopy )

The secretory pole of vertebrate oxyntic cells possesses two distinct membrane systems: the apical plasma membrane which presents numerous infoldings, microvilli and processes, and a complex tubulovesicular system located in close proximity to the plasma membrane. These two membrane systems are generally believed to be interconvertible in relation to the functional state of the cell. To determi...

Phalloidin-induced proliferation of actin filaments within rat hepatocytes. Visualisation by electron microscopy and immunofluorescence.

Confirming our previous ultrastructural and biochemical studies, the combined electron microscopy and immunofluorescence investigations show that phalloidin causes a characteristically striking increase of actin filaments within rat hepatocytes. Filaments are localized mainly at the level of special junctional complexes between contiguous cells and around bile canaliculi.

Direct measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores

We used actin filament bundles isolated from intestinal brush-border microvilli to nucleate the polymerization of pure muscle actin monomers into filaments. Growth rates were determined by electron microscopy by measuring the change in the length of the filaments as a function of time. The linear dependence of the growth rates on the actin monomer concentration provided the rate constants for m...

Improved Preservation of Parotid Tissue for Electron Microscopy

Parotid tissue is, in many respects, the best subject for studies on the genesis of exportable proteins and on the mechanism of exocrine secretion . It has been adopted by us for combined biochemical, radioautographic, and morphometric studies on the production and fate of zymogen granules . To our knowledge, however, no detailed stereological analyses of parotid tissue have been carried out at...

Electron Microscopy of Fowl Pox Virus